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KMID : 0363320090300010036
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Journal of Korean Oriental Internal Medicine
2009 Volume.30 No. 1 p.36 ~ p.50
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The Effects of Lycium Chinense Milie on the H2O2-treated LLC-PK₁Cell’s Redox Status and NF-κB Signaling
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Choi Gyu-Ho
Shin Hyeon-Cheol
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Abstract
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Objectives: This study was aimed to verify the cytoprotective function, antioxidative effect and inflammation genes inhibitory effects of Lycium chinense Milie. Therefore the generation of superoxide anion radical (O₂-), peroxynitrite (ONOO-), nitric oxide (NO) and prostaglandin E₂(PGE₂)was investigated in the renal epithelial cells of mouse. Effects of Lycium chinense Milie on the expression of inflammation-related proteins, IKK-α, p-IKK-αβ, p-IκB-α, NF-κB (p50, p65), COX-2 and iNOS, were examined by western blotting.
Methods: For this study, the fluorescent probes were used, namely dihydrorhodamine 123 (DHR 123), 4.5-diaminofluorescein (DAF-2) and 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA). Western blotting was performed using anti-IKK-α, anti-phospho IKK-αβ, anti-phospho IκB-α, anti-NF-κB (p50, p65), anti-COX-2 and anti-iNOS, respectively.
Results: Lyciutn chinense Milie reduced H2O2-induced cell death dose-dependently. It inhibited the generation of O₂-, ONOO-, NO and PGE₂in the H2O2-treated renal epithelial cells of mouse in vitro. Lycium chinense Milie inhibited the expression of IKK-α, p-IKK-αβ, p-IκB-α, COX-2 and iNOS genes by means of decreasing activation of NF-κB.
Conclusions: According to above results. Lycium chinense Milie recommended to be applied in treatment for the inflammatory process and inflammation-related diseases.
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KEYWORD
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Lvcium chinense Milie, LLC-PK₁cell, H₂O₂, O₂-, ONOO- , NO, PGE₂ , NF-κB, COX-₂, iNOS
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